Composite

Part:BBa_K2683014:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge   (2018-10-08)


FitD Codon optimized for E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2601
    Illegal BglII site found at 2793
    Illegal BamHI site found at 2247
    Illegal BamHI site found at 2349
    Illegal BamHI site found at 2880
    Illegal BamHI site found at 4362
    Illegal BamHI site found at 6534
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 507
    Illegal NgoMIV site found at 2040
    Illegal NgoMIV site found at 2766
    Illegal NgoMIV site found at 2946
    Illegal NgoMIV site found at 3051
    Illegal NgoMIV site found at 3099
    Illegal NgoMIV site found at 4710
    Illegal AgeI site found at 3786
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As FitD has a relatively large coding sequence, special considerations for assembly was done. In order to get the entire sequence in the form of g-blocks, the sequences were designed into eight separate g-blocks that would be assembled by SLIC assembly.


Source

Boston, M. (2018). US Patent No. 0139968 A1. Washington, DC: U.S. Patent and Trademark Office.

References

[1] Molloy, D., Mayer, D., Giamberini, L and Gaylo, M. (2013) Mode of action of speudomonas fluorescens strain CL145A, a lethal control agent of Dreissend mussels (Bivalvaia: Dreissenidae). JOurnal of invertebrate pathology. 113, 115-121